Activated Prothrombin Time: Definition, Procedure, Normal Range, Uses

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Activated Prothrombin Time (APTT):

Activated prothrombin time test is a type of blood test used to measure the time taken for the citrated plasma to clot in the presence of a surface activator, calcium chloride, and phospholipid.

Also Read:

• Bleeding Disorders
• Clotting Time, Methods, Procedure Normal Range, And Uses

Materials required:

• Test Tube (Vaccutainer)
• Patient's Plasma (Obtainted by centrifugation of blood sampe)
• Anticoagulant
• Surface Activator
• Phospholipid
• Calcium chloride
• Sterile Glass Stirrer
• Optical Or Mechanical Instrument
• Stopwatch.

Procedure Of Prothrombin Time Test:

1. Collect the patient's blood sample in a clean vacctainer containing anticoagulant.
2. Centriguge the blood sample under certain rpm in given time 3-5 minutes.
3. Then separate the supernatant plasma from the whole blood in a separate test tube.
4. Take 0.1 ml of plasma in a sterile test tube and add 0.1 ml of surface activator kaolin to it and mix it throughly.
5. Further, the mixture should be placed in a water bath for 10 minutes at 37 degree centigrade.
6. Then in other test tube mix 2 ml of calcium chloride solution and 2 ml phospholipids suspension and incubate in a water bath for 10 minutes at 37 degree centigrade.
7. Take 0.2 ml of mixture of phospholipid and calcium chloride into the test tube containing mixture of plasma and surface activator kaolin,
8. Stir the mixture thoroughly and then immediately on the stop watch.
9. Then note the time interval for the mixture to clot.

Normal range of prothrombin test: 30-40 seconds.

Also Read:

• Pheripheral Blood Smear
• Bleeding Time: Procedure, Normal Range, And Uses

Uses Of Prothrombin Test:

• It is used to monitor the heparin therapy.
• It is used as screening test to detect bleeding disorders due to deficiency of the coagulation factors such as factor II, VIII, IX, X, XI, and XII.
• It helps the physician to diagnose and direct the treatment for the disorder.

Also Read:

• Thrombin Time
• Activated Prothrombin Time (APTT)

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