Prothrombin Time (PTT): Defintion, Procedure, Normal Range, Uses

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Prothrombin Time (PTT):

Prothrombin time is the time taken for clot formation in the citrate plama sample after theaddition of tissue thromboplastin and calcium chloride solution.

Also Read:

• Pheripheral Blood Smear
• Thrombin Time

Materials required:

• Patient's Plasma (Obtainted by centrifugation of blood sampe)
• Test Tube (Vaccutainer)
• Anticoagulant
• Tissue thromboplastin
• Calcium chloride
• Sterile Glass Stirrer
• Optical Or Mechanical Instrument
• Stopwatch.

Procedure Of Prothrombin Time Test:

1. First step is the collection of atient's blood in a clean sterile vaccutainer containing anticoagulant.
2. Then place the blood sample in a centrifugation machine.
3. After centrifugation, separate the supernatant plasma from the whole blood in to other test tube.
4. Incubate all the test tubes and reagents used for the procedure for 15 minutes at 37 degree centrigrade.
5. Then take 0.1 ml of plasma in a test tube and add 0.1 ml of tissue thromboplastic suspension and incubate it for 2 minutes at 37 degree centrigrade.
6. Add 0.1 ml of calcium chloride solution to the mixture and stir it thoroughly.
7. Then immediately on the sto watch and leave the test tube in a water bath for 8-9 seconds.
8. Notice any clot formation in te test tube by tilting it gently.
9. Record the time soon after clot formation in the test tube.

Normal range of prothrombin test: 11-16 seconds.

Also Read:

• Bleeding Disorders
• Clotting Time, Methods, Procedure Normal Range, And Uses

Uses Of Prothrombin Test:

• It is used to monitor the antigualant therapy.
• To detect hepatic functions.
• Increased prothrombin time indicates liver disease and vitamin K deficiency.
• Prothrombin test is common test to diagnose the coagulantion disorders that are caused due to deficiency of clotting factors such as factor I, II, V, VII, and X.

Also Read:

• Bleeding Time: Normal Range, Procedure, And Uses
• Activated Prothrombin Time (APTT)

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